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KMID : 1094720230280050790
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Biotechnology and Bioprocess Engineering
2023 Volume.28 No. 5 p.790 ~ p.803
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Co-utilization of Maltose and Sodium Acetate via Engineered Corynebacterium glutamicum for Improved Itaconic Acid Production
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Taghreed Elkasaby
Dao Duy Hanh
Hideo Kawaguchi
Masakazu Toyoshima
Akihiko Kondo
Chiaki Ogino
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Abstract
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For growth and energy, Corynebacterium glutamicum has the ability to assimilate numerous carbon sources in the form of either single or combined substrates. During the growth of C. glutamicum on substrate mixtures, it has shown the ability to co-metabolize the majority of these carbon sources and displays monophasic growth, unlike other microorganisms such as Escherichia coli and Bacillus subtilis, which exhibit either diauxic or biphasic growth. Here, a recombinant strain of C. glutamicum ATCC 13032 was selected for the use in the production of itaconic acid (IA), which is a promising biochemical building block that could be an alternative material for polymer synthesis. For this purpose, an engineered C. glutamicum ATCC 13032 pCH-cadAopt was constructed by introducing the plasmid pCH-cadAopt, which expressed a cis-aconitate dehydrogenase gene (cadA) that originated from Aspergillus terreus. The production of IA was evaluated using a combined mixture of maltose and sodium acetate. The monophasic growth of C. glutamicum in the presence of maltose and sodium acetate was observed and showed final IA titer of 12.63 g/L, and a molar yield of 0.38 mol/mol after 240 h of cultivation. The present study suggests the possibility of utilizing a mixture of carbon sources by C. glutamicum to improve IA production.
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KEYWORD
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itaconic acid Corynebacterium glutamicum, sodium acetate, monophasic growth, flux balance analysis
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